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Molecular Biology

Molecular
Biology

Molecular Biology in Current Medicine


It has been more than 150 years to better understand the complex way of how living beings on earth store their genetic information and how such information is able to interact with cellular structures to create individuals as complex as human beings.

Molecular biology is very useful to understand, diagnose and predict most frequent diseases suffered by human race.

Molecular biology in today's medicine

Techniques Used


Cytochemistry

Analysis of chemical composition of cell substances and their location.

Cell culture

Fluorescence in situ hybridization –FISH
   • Cell lysis

   • Fixation of nuclei

   • Denaturation / Hybridization

   • Reading

FISH advantages
   • Specificity

   • Sensitivity for cell analysis

   • Multiplicity of cell material

   • Detection of chromosomal abnormalities

Cytochemistry
 

Comparative Genomic Hybridization –CGH

A technique that allows simultaneous exploration of multiple genome areas by means of using DNA microchips (microarrays).

CGH Array
   • Fragmentation
   • Purification
   • Hybridization

   • Reading

CGH Advantages
   • Specificity

   • Promptness

   • Sensitivity to analyze DNA fragments

   • Multiplicity of tissues and fluids

   • High-resolution chromosome analysis

Comparative Genomic Hybridization –CGH
 

Polymerase Chain Reaction –PCR

The polymerase chain reaction (PCR) technique is an in vitro method in which an enzymatic synthesis of a specific DNA fragment is produced in a few hours.

PCR Conventional
   • Purification

   • Amplification

   • Electrophoresis

   • Reading

PCR Quantitative
   • Purification
   • Amplification
   • Reading

PCR Advantages
   • Specificity

   • Sensitivity to determine nucleic acids

   • Promptness

   • Multiplicity of tissues and fluids

   • Quantification of charges

   • Multiplex tailored

   • Mutation detection

Polymerase Chain Reaction –PCR